Subfamily Anophelinae
- Genus Anopheles Meigen, 1818
- Subgenus Anopheles Meigen, 1818
- Anopheles annulipalpis Lynch Arribálzaga, 1878
- Anopheles apicimacula Dyar and Knab, 1906
- Anopheles argentinus (Brèthes, 1912)
- Anopheles evandroi Da Costa Lima, 1937
- Anopheles fluminensis Root, 1927
- Anopheles maculipes (Theobald, 1903)
- Anopheles medialis Harbach, 2018
- Anopheles mediopunctatus (Lutz, 1903)
- Anopheles minor Da Costa Lima, 1929
- Anopheles neomaculipalpus Curry, 1931
- Anopheles punctimacula Dyar and Knab, 1906
- Anopheles tibiamaculatus (Neiva, 1906)
- Genus Chagasia Cruz, 1906
- Chagasia fajardi (Lutz, 1904)
- Genus Kerteszia Theobald, 1905
- Kerteszia bambusicola (Komp, 1937)
- Kerteszia cruzii (Dyar and Knab, 1908)
- Kerteszia laneanus (Correa and Cerqueira, 1944)
- Genus Nyssorhynchus Blanchard, 1902
- Subgenus Myzorhynchella Theobald, 1907
- Nyssorhynchus antunesi (Galvao and Amaral, 1940)
- Nyssorhynchus guarani (Shannon, 1928)
- Nyssorhynchus lutzii (Cruz, 1901)
- Nyssorhynchus nigritarsis (Chagas, 1907)
- Subgenus Nyssorhynchus Blanchard, 1902
- Nyssorhynchus marajoara (Galvão and Damasceno, 1942)
- Nyssorhynchus albitarsis (Lynch Arribálzaga, 1878)
- Nyssorhynchus argyritarsis (Robineau-Desvoidy, 1827)
- Nyssorhynchus braziliensis (Chagas, 1907)
- Nyssorhynchus darlingi (Root, 1926)
- Nyssorhynchus deaneorum (Rosa-Freitas, 1989)
- Nyssorhynchus evansae (Brèthes, 1926)
- Nyssorhynchus galvaoi (Causey, Deane and Deane, 1943)
- Nyssorhynchus nuneztovari (Gabaldon, 1940)
- Nyssorhynchus oswaldoi (Peryassú, 1922)
- Nyssorhynchus rangeli (Gabaldon, Cova-García and López, 1940)
- Nyssorhynchus rondoni (Neiva and Pinto, 1922)
- Nyssorhynchus strodei (Root, 1926)
- Nyssorhynchus triannulatus (Neiva and Pinto, 1922)
- Genus Unnamed
- Anopheles parvus (Chagas, 1907)
