• Genus Isostomyia Coquillett, 1906
• Isostomyia espini (Martini, 1914)
• Isostomyia paranensis (Bèthes, 1910)

• Genus Limatus Theobald, 1901
• Limatus durhamii Theobald, 1901

• Genus Onirion Harbach and Peyton, 2000
• Onirion brucei (Del Ponte and Cerqueira, 1938)
• Onirion personatum (Lutz, 1904)

• Genus Runchomyia Theobald, 1903
• Subgenus Runchomyia Theobald, 1903
• Runchomyia frontosa Theobald, 1903
• Runchomyia reversa (Lane and Cerqueira, 1942)

• Genus Sabethes Robineau-Desvoidy, 1827
• Subgenus Davismyia Lane and Cerqueira, 1942
• Sabethes petrocchiae (Shannon and Del Ponte, 1928)
• Subgenus Peytonulus Harbach, 1991
• Sabethes aurescens (Lutz, 1905)
• Sabethes identicus Dyar and Knab, 1907
• Sabethes soperi Lane and Cerqueira, 1942
• Sabethes undosus (Coquillett, 1906)
• Subgenus Sabethes Robineau-Desvoidy, 1827
• Sabethes albiprivus Theobald, 1903
• Sabethes belisarioi Neiva, 1908
• Sabethes cyaneus (Fabricius, 1805)
• Sabethes purpureus (Theobald, 1907)
• Subgenus Sabethinus Lutz, 1904
• Sabethes intermedius (Lutz, 1904)
• Sabethes melanonymphe Dyar, 1924
• Subgenus Sabethoides Theobald, 1903
• Sabethes chloropterus (von Humboldt, 1819)
• Sabethes glaucodaemon (Dyar & Shannon, 1925)

• Genus Shannoniana Lane and Cerqueira, 1942
• Shannoniana fluviatilis (Theobald, 1903)

• Genus Trichoprosopon Theobald, 1901
• Trichoprosopon castroi Lane and Cerqueira, 1942
• Trichoprosopon compressum Lutz, 1905
• Trichoprosopon obscurum Lane and Cerqueira, 1942
• Trichoprosopon pallidiventer (Lutz, 1905)
• Trichoprosopon simile Lane and Cerqueira

• Genus Wyeomyia Theobald, 1901
• Wyeomyia melanocephala Dyar and Knab, 1906
• Wyeomyia serratoria Dyar and Nuñez Tovar, 1927
• Subgenus Dendromyia Theobald, 1903
• Wyeomyia luteoventralis Theobald, 1901
• Subgenus Dodecamyia Dyar, 1918
• Wyeomyia aphobema Dyar, 1918
• Subgenus Menolepis Lutz, 1908
• Wyeomyia leucostigma Lutz, 1904
• Subgenus Miamyia Dyar, 1919
• Wyeomyia codiocampa Dyar and Knab, 1907
• Wyeomyia limai Lane and Cerqueira, 1942
• Wyeomyia lutzi (Da Costa Lima, 1930)
• Wyeomyia oblita (Lutz, 1904)
• Wyeomyia sabethea Lane and Cerqueira, 1942
• Wyeomyia serrata (Lutz, 1905)
• Subgenus Nunezia Dyar, 1928
• Wyeomyia lateralis Petrocchi, 1927
• Subgenus Phoniomyia Theobald, 1903
• Wyeomyia diabolica (Lane and Forattini, 1952)
• Wyeomyia flabellata (Lane and Cerqueira, 1942)
• Wyeomyia fuscipes Edwards, 1922
• Wyeomyia muehlensi Petrocchi, 1927
• Wyeomyia pilicauda Root, 1928
• Wyeomyia quasilongirostris (Theobald, 1907)
• Wyeomyia tripartita (Bonne-Wepster and Bonne, 1921)
• Subgenus Spilonympha Motta and Lourenço-de-Oliveira, 2005
• Wyeomyia mystes Dyar, 1924
• Subgenus Triamyia Theobald, 1919
• Wyeomyia aporonoma Dyar and Knab, 1906
• Subgenus Wyeomyia Theobald, 1901
• Wyeomyia arthrostigma (Lutz, 1905)
• Wyeomyia medioalbipes Lutz, 1904