Belkin et al. (1970) suggested that Mansonia titillans populations in the northern and southern parts of its range may be different (Mitchell & Darsie, 1985).
Disease relations: Specimens of Mansonia titillans were reported naturally infected with the Saint Louis Encephalitis virus in Córdoba, Colombia (Hoyos-López et al., 2015). Western Equine Encephalitis virus has been isolated from specimens identified as Mansonia spp, in Santa Fe Province between 1982 and 1983 (Sabattini et al., 1998).
Eggs are usually adhered to aquatic plants and larvae get air from them. Mansonia titillans have been collected with Coquillettidia juxtamansonia, Coquillettidia albicosta, Coquillettidia venezuelensis and Mansonia pseudotitillans sharing the same breeding places. Adults are large, aggressive biters and mostly zoophilic, but opportunistic in their preferences.